Fig. 3

Effect of the reaction pH for free and immobilized enzyme. a Effect on the BFA/FA molar ratio. b Effect on the BFA specific activity. (Reactions were performed in a 1-butanol (92.5%) phosphate-citrate buffer (7.5%) mixture. The pH of the buffer fraction was varied from 6.0 to 8.0. Immobilization was performed at pH 6.5 and only the reaction pH was varied.) All reactions were in initial velocity. The results are the average of triplicate samples, and the error bars represent one standard deviation