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Table 1 Mutations of selected epRCA transformants

From: Effect of mutations to amino acid A301 and F361 in thermostability and catalytic activity of the β-galactosidase from Bacillus subtilis VTCC-DVN-12-01

Transformant RA (%) Nucleotide substitution Amino acid substitution
Wild-type 100 original  
LacA125 167 No  
LacA221 150 No  
LacA887 198 ACG → ACA, CGG → CGA T408, R624
LacA259 52 UUC → UAU, GCU → ACU F361Y, A524T
LacA461 36 GAG → GAA, CGG → UGG, GCG → GUG, GCG → GUG E60, R77W, A191V, A301V
  1. Plasmids from selected colonies were isolated and sequenced to identify mutations in the lacA gene
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BMC Biochemistry

ISSN: 1471-2091

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