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Table 2 Kinetic parameters of purified wild-type and mutant CsnN174

From: A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding

Enzyme Km(μg/ml) kcat(min-1) Ki(μg/ml) kcat/ Km(min*ml/μg)
Wild-type 26.1 642.1 1547 24.6
R42E 441.7 96.7 263 0.22
R42K 204.1 55.5 612 0.27
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BMC Biochemistry

ISSN: 1471-2091

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