A highly conserved arginine residue of the chitosanase from Streptomyces sp. N174 is involved both in catalysis and substrate binding

Table 1 Specific activities of purified wild-type and mutant CsnN174

Enzyme	Specific activity (units per mg protein)
Wild type*	52.9
D40G*	1.6
R42E	0.9
R42K	1.4
D40G+R42E	0.0012
D40G+R42K	0.018

ISSN: 1471-2091